WebINVSC1酿酒酵母可在YPD培养基上生长,用电转化的方法可将质粒转入酵母细胞中,用半乳糖诱导蛋白表达。. 用30%的甘油与培养物混合可保藏菌种。. 操作说明:. 1,本品包含一份甘油菌,使用本甘油菌时可以不用完全融解,在甘油菌表面蘸取少量涂板或进行液体 ... Web12 okt. 2013 · Aspergillus niger glucose oxidase (GOx) genes for wild-type (GenBank accession no. X16061, swiss-Prot; P13006) and M12 mutant (N2Y, K13E, T30 V, I94 V, K152R) were cloned into pPICZαA vector for expression in Pichia pastoris KM71H strain. The highest expression level of 17.5 U/mL of fermentation media was obtained in 0.5 % …
Protein Expression In Yeast Using INVSc1 And pYES2/CT From ..…
Web1 apr. 2024 · INVSc1; δ1-P HHF2-HMG1 P IRA1- ... Strain dependence for squalene accumulation from engineered S. cerevisiae strains overexpressing tHMG1 and ispA. Using the new protocol described here, the strains employed for testing basal squalene levels were evaluated for their potential use as hosts for squalene accumulation following ... Webvi Product Qualification Introduction This section describes the criteria used to qualify the pYES6/CT and pYC6/CT vectors and the INVSc1 yeast strain. Vectors Each vector is qualified by restriction enzyme digestion with specific restriction enzymes as listed below. Restriction digests must demonstrate the correct banding pattern when optimus cleaning services
INVSc1,酿酒酵母酵母菌菌株
WebA three-plasmid yeast expression system utilizing the portable small ubiquitin-like modifier (SUMO) vector set combined with the efficient endogenous yeast protease Ulp1 was … Web[0033] In order to show the coding function of the RrFAE1 gene, the present invention clones the RrFAE1 gene between the BamHI and KpnI sites of pYES2/NT C of Invitrogen, and transforms the yeast strain InvSc1 to obtain high expression. Web4 jan. 2024 · Approximately 200 ng of plasmid p414-TEF 1p-Cas9-CYC1t (DiCarlo et al., 2013) was used to transform yeast strain InvSc1 [MATa his3Δ1 leu2 trp1-289 ura3-52 MATα his3Δ1 leu2 trp1-289 ura3-52] to obtain yeast strain InvSc1-Cas9. portland state university grade scale